Human CYBA KO Cell Line-HEK293T

Name: Human CYBA KO Cell Line-HEK293T
SKU: CSC-RT2853
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2853

Target Gene : CYBA Host Cell : HEK293T

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2853
Description	This cell is a stable cell line with a homozygous knockout of human CYBA using CRISPR/Cas9.
Target Gene	CYBA
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CYBA cytochrome b-245, alpha polypeptide [ Homo sapiens ]
Gene Symbol	CYBA
Synonyms	CYBA; cytochrome b-245, alpha polypeptide; cytochrome b-245 light chain; flavocytochrome b 558 alpha polypeptide; p22 PHOX; p22phox; cytochrome b light chain; p22 phagocyte B-cytochrome; cytochrome b alpha chain; cytochrome b558 subunit alpha; cytochrome b alpha-subunit; cytochrome b, alpha polypeptide; flavocytochrome b-558 alpha polypeptide; neutrophil cytochrome b 22 kDa polypeptide; superoxide-generating NADPH oxidase light chain subunit; p22-PHOX; FLJ43590; FLJ99071;
GeneID	1535
Uni ProtID	P13498
mRNA Refseq	BC028224
Chromosome Location	16q24
Function	SH3 domain binding; electron carrier activity; electron carrier activity; heme binding; metal ion binding; oxidoreductase activity; protein binding; protein heterodimerization activity; contributes_to superoxide-generating NADPH oxidase activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Antigen processing-Cross presentation, organism-specific biosystem; Class I MHC mediated antigen processing and presentation, organism-specific biosystem; Cross-presentation of particulate exogenous antigens (phagosomes), organism-specific biosystem; Immune System, organism-specific biosystem; Leishmaniasis, organism-specific biosystem;

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