Human CSTF2 KO Cell Line-HeLa

Name: Human CSTF2 KO Cell Line-HeLa
SKU: CSC-RT2953
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2953

Target Gene : CSTF2 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2953
Description	This cell is a stable cell line with a homozygous knockout of human CSTF2 using CRISPR/Cas9
Target Gene	CSTF2
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CSTF2 cleavage stimulation factor, 3 pre-RNA, subunit 2, 64kDa [ Homo sapiens ]
Gene Symbol	CSTF2
Synonyms	CstF-64
Gene Description	cleavage stimulation factor, 3 pre-RNA, subunit 2, 64kDa
Gene ID	1478
Uni Prot ID	P33240
m RNA Refseq	NM_001325.2
Protein Refseq	NP_001316.1
Chromosome Location	Xq22.1
Function	RNA binding; nucleotide binding; protein binding;
Pathway	Cleavage of Growing Transcript in the Termination Region, organism-specific biosystem; Gene Expression, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; Processing of Capped Intronless Pre-mRNA, organism-specific biosystem; Processing of Intronless Pre-mRNAs, organism-specific biosystem; RNA Polymerase II Transcription, organism-specific biosystem; RNA Polymerase II Transcription Termination, organism-specific biosystem;
MIM	600368

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