Human COL12A1 KO Cell Line-Hela

Name: Human COL12A1 KO Cell Line-Hela
SKU: CSC-RT2877
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2877

Target Gene : COL12A1 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2877
Description	This cell is a stable cell line with a homozygous knockout of human COL12A1 using CRISPR/Cas9.
Target Gene	COL12A1
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	COL12A1 collagen, type XII, alpha 1 [ Homo sapiens ]
Gene Symbol	COL12A1
Synonyms	COL12A1L; BA209D8.1; DJ234P15.1
Gene Description	collagen, type XII, alpha 1
Gene ID	1303
Uni Prot ID	Q99715
m RNA Refseq	NM_080645.2
Protein Refseq	NP_542376.2
Chromosome Location	6q12-q13
Function	extracellular matrix structural constituent conferring tensile strength;
Pathway	Collagen biosynthesis and modifying enzymes, organism-specific biosystem; Collagen formation, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem;
MIM	120320

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