Human CISD1 KO Cell Line-HeLa

Name: Human CISD1 KO Cell Line-HeLa
SKU: CSC-RT2935
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2935

Target Gene : CISD1 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2935
Description	This cell is a stable cell line with a homozygous knockout of human CISD1 using CRISPR/Cas9
Target Gene	CISD1
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CISD1 CDGSH iron sulfur domain 1 [ Homo sapiens ]
Gene Symbol	CISD1
Synonyms	CISD1; CDGSH iron sulfur domain 1; C10orf70, chromosome 10 open reading frame 70 , ZCD1, zinc finger, CDGSH type domain 1; CDGSH iron-sulfur domain-containing protein 1; MDS029; mitoNEET; zinc finger CDGSH-type domain 1; zinc finger, CDGSH-type domain 1; CDGSH iron sulfur domain-containing protein 1; ZCD1; C10orf70; MGC14684;
GeneID	55847
Uni ProtID	Q9NZ45
mRNA Refseq	BC005962
Chromosome Location	10q21.3
Function	2 iron, 2 sulfur cluster binding; metal ion binding;
Pathway	Adipogenesis, organism-specific biosystem;
MIM	611932

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