Human CDK12 KO Cell Line-HeLa

Name: Human CDK12 KO Cell Line-HeLa
SKU: CSC-RT2973
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2973

Target Gene : CDK12 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2973
Description	This cell is a stable cell line with a homozygous knockout of human CDK12 using CRISPR/Cas9
Target Gene	CDK12
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CDK12 cyclin-dependent kinase 12 [ Homo sapiens ]
Gene Symbol	CDK12
Synonyms	CRK7; CRKR; CRKRS; hCDK12
GeneID	51755
Uni ProtID	Q9NYV4
mRNA Refseq	NM_016507.2
Protein Refseq	NP_057591.2
Chromosome Location	17q12
Function	ATP binding; RNA polymerase II carboxy-terminal domain kinase activity; cyclin binding; cyclin-dependent protein kinase activity; protein binding; protein kinase activity;

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