Human CAPN1 Knockout Cell Line-HEK293T

Name: Human CAPN1 Knockout Cell Line-HEK293T
SKU: CSC-RT2810
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2810

Target Gene : CAPN1 Host Cell : HEK293T

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2810
Description	This cell is a stable cell line with a homozygous knockout of human CAPN1 using CRISPR/Cas9.
Target Gene	CAPN1
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CAPN1 calpain 1, (mu/I) large subunit [ Homo sapiens ]
Gene Symbol	CAPN1
Synonyms	CAPN1; calpain 1, (mu/I) large subunit; calpain-1 catalytic subunit; CANP; CANPL1; muCANP; muCL; CANP 1; calpain mu-type; micromolar-calpain; calpain-1 large subunit; calpain, large polypeptide L1; calcium-activated neutral proteinase 1; cell proliferation-inducing protein 30; cell proliferation-inducing gene 30 protein; CANP1;
GeneID	823
Uni ProtID	P07384
mRNA Refseq	BC017200
Chromosome Location	11q13
Function	calcium ion binding; calcium-dependent cysteine-type endopeptidase activity; peptidase activity; protein binding;
Pathway	Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Focal Adhesion, organism-specific biosystem; Integrin-mediated cell adhesion, organism-specific biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem;
MIM	114220

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