Human ALKBH5 KO Cell Line-HEK293T

Name: Human ALKBH5 KO Cell Line-HEK293T
SKU: CSC-RT2919
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2919

Target Gene : ALKBH5 Host Cell : HEK293T

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2919
Description	This cell is a stable cell line with a homozygous knockout of human ALKBH5 using CRISPR/Cas9
Target Gene	ALKBH5
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ALKBH5 alkB, alkylation repair homolog 5 (E. coli) [ Homo sapiens ]
Gene Symbol	ALKBH5
Synonyms	ABH5; OFOXD; OFOXD1
Gene Description	alkB, alkylation repair homolog 5 (E. coli)
Gene ID	54890
Uni Prot ID	Q6P6C2
m RNA Refseq	NM_017758.3
Protein Refseq	NP_060228.3
Chromosome Location	17p11.2; 17p11.2
Function	metal ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen;
MIM	613303

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