Human ROCK1 Knockdown Cell Line - MCF7

Name: Human ROCK1 Knockdown Cell Line - MCF7
SKU: CSC-DC013461-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC013461-1

Host Cell : MCF7 Validation : Real-Time RCR

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Cell Line Information

Safety and Packaging

Gene Information

Cat. No.	CSC-DC013461-1
Description	This cell line is engineered to stably overexpress shRNA targeting human ROCK1. Expression of human ROCK1 gene is stably knocked down in this cell line.
Target Gene	ROCK1
Host Cell	MCF7
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Storage	Liquid nitrogen
Shipping	Dry ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ROCK1 Rho-associated, coiled-coil containing protein kinase 1 [ Homo sapiens ]
Gene Symbol	ROCK1
Synonyms	ROCK-I; P160ROCK
Gene Description	Rho-associated, coiled-coil containing protein kinase 1
GeneID	6093
Uni ProtID	Q13464
mRNA Refseq	NM_005406.2
Protein Refseq	NP_005397.1
Chromosome Location	18q11.1
Pathway	Apoptosis, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem; Apoptotic execution phase, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem;
MIM	601702

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Background

Case Study

Applications

Q & A

Customer Reviews

The ROCK1 gene, also known as Rho-associated protein kinase 1, encodes a serine/threonine protein kinase that is involved in various cellular processes, including cell migration, proliferation, and differentiation. It is a key regulator of actin cytoskeleton organization and plays a crucial role in maintaining cell shape and adhesion. Dysregulation of the ROCK1 gene has been associated with several diseases, such as fibrosis and cancer.

MCF7 Cell Line is a breast cancer cell line that was derived from the pleural effusion of a 55-year-old woman with estrogen-receptor-positive adenocarcinoma. MCF7 cells are widely used in research for studying the biology of breast cancer, particularly the effects of hormones and anti-hormonal therapies. They have been extensively characterized and are known for their ability to grow in vitro and respond to estrogen stimulation. MCF7 cells are also a valuable tool for testing the efficacy of potential anti-cancer drugs and for understanding the molecular mechanisms of breast cancer development and progression.

Rho-associated kinase 1(ROCK1)regulates tumour metastasis by maintaining cytoskeletal homeostasis.Some researchers have investigated the function of ROCK1 in NSCLS survival using RNA interference-mediated knockdown.The results showed that ROCK1 knockdown reduced cell survival in vitro.It also inhibited cell migration and proliferation.Transfection of ROCK1 siRNA was associated with increased expression of large tumour suppressor kinase 2(LATS2)and c-Jun N-terminal kinase(JNK).In addition,ROCK1 knockdown-induced inhibition of apoptosis and proliferation was suppressed by LATS2 knockdown or JNK inactivation,suggesting that ROCK1 deficiency triggers apoptosis in a LATS2-JNK pathway-dependent manner.Functional analyses further showed that ROCK1 knockdown leads to dysregulation of mitochondrial dynamics and inhibits mitochondrial biogenesis.This effect was also reversed by LATS2 knockdown or JNK inactivation.

Figure 1. ROCK1 knockdown decreases cell migration and proliferation. Figure 1.ROCK1 knockdown decreases cell migration and proliferation.(A)A CCK-8 assay was used to quantify proliferation in ROCK1 knockdown cells or control cells.(B,C)A qPCR assay was used to analyze Cyclin-D and Cyclin-E transcription.(D)Transwell assay for ROCK1 knockdown cells.(E,F)A qPCR assay was used to analyze CXCR-4 and CXCCR-7 transcription.(Xin,Tinget al.2020)

The Human ROCK1 Knockdown MCF7 Cell Line is a cellular model that has been developed to study the role of ROCK1 (Rho-associated kinase 1) in breast cancer progression.

In scientific research, this cell line allows researchers to explore the mechanisms by which ROCK1 contributes to breast cancer metastasis and to test the efficacy of potential inhibitors of ROCK1. The findings from these studies can contribute to a better understanding of the role of ROCK1 in breast cancer and potentially lead to the development of novel therapeutics targeting this protein.

In clinical settings, the Human ROCK1 Knockdown MCF7 Cell Line can be used to study the effects of ROCK1 inhibitors on breast cancer cells, which can help in the selection of patients who may benefit from these therapies. Additionally, this cell line can be used to assess the potential side effects of ROCK1 inhibitors and to optimize dosing regimens.

In the context of translational research, the cell line can be used to develop preclinical models of breast cancer that more closely resemble the disease in humans. This can aid in the translation of basic research findings into clinical applications, facilitating the development of new treatments for breast cancer patients.

Overall, the Human ROCK1 Knockdown MCF7 Cell Line is a valuable tool that is contributing to advancements in the understanding, treatment, and prevention of breast cancer, with the potential to improve outcomes for patients with this disease.

Customer Q&As

What is the ROCK1 gene and its function?

A: The ROCK1 gene encodes for the Rho-associated coiled-coil kinase 1, which is a serine/threonine kinase. ROCK1 plays a critical role in regulating the actin cytoskeleton, controlling cell motility, proliferation, and apoptosis. It is involved in various cellular processes, including smooth muscle contraction, cell adhesion, and epithelial-to-mesenchymal transition.

How does the ROCK1 gene contribute to disease development?

A: The ROCK1 gene contributes to disease development by its involvement in the regulation of cell processes. Dysregulation of ROCK1 has been linked to various conditions, including fibrosis, cancer metastasis, and cardiovascular diseases. Increased ROCK1 activity can promote aberrant cell behavior, leading to the progression of these diseases.

Are there any therapeutic implications of targeting the ROCK1 gene?

A: Targeting the ROCK1 gene has therapeutic implications for diseases where its dysregulation is a key factor. Inhibitors of ROCK1 activity can be used to treat conditions such as fibrosis, cancer metastasis, and hypertension. These targeted therapies can modulate ROCK1-dependent processes and potentially reverse or prevent the progression of these diseases.

How is the expression of the ROCK1 gene regulated?

A: The expression of the ROCK1 gene is regulated by various factors, including extracellular signals, cytokines, and transcription factors. It can be activated by Rho GTPases, such as RhoA, which are involved in cell signaling pathways. The activity of ROCK1 can also be influenced by post-translational modifications, such as phosphorylation, and its subcellular localization.

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Customer Reviews

Repeatability

The Human ROCK1 Knockdown Cell Line - MCF7 consistently demonstrates reliable and consistent results across various experiments, ensuring the validity and reproducibility of research findings.

United Kingdom

2023-06-28

Cost-effectiveness

The Human ROCK1 Knockdown Cell Line - MCF7 offers a cost-effective solution for researchers, providing a valuable tool for studying ROCK1 protein function without the need for expensive and time-consuming knockout techniques.

Germany

2020-12-28

Market feedback

Researchers who have utilized the Human ROCK1 Knockdown Cell Line - MCF7 have reported positive feedback, highlighting its effectiveness in studying ROCK1-related cellular processes and its potential applications in drug discovery and development.

United States

2021-08-03

After-sales service

The provider of the Human ROCK1 Knockdown Cell Line - MCF7 offers excellent after-sales support, including detailed documentation, technical assistance, and prompt responses to inquiries, ensuring a smooth and hassle-free experience for researchers.

French

2022-05-26

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Human ROCK1 Knockdown Cell Line - MCF7

Cell Line Information

Safety and Packaging

Gene Information

Background

Case Study

Applications

Q & A

Customer Reviews

What is the ROCK1 gene and its function?

How does the ROCK1 gene contribute to disease development?

Are there any therapeutic implications of targeting the ROCK1 gene?

How is the expression of the ROCK1 gene regulated?

Repeatability

Cost-effectiveness

Market feedback

After-sales service

Related Products

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