Cat.No. : CSC-DC013461-1
Host Cell: MCF7 Validation: Real-Time RCR
| Cat. No. | CSC-DC013461-1 |
| Description | This cell line is engineered to stably overexpress shRNA targeting human ROCK1. Expression of human ROCK1 gene is stably knocked down in this cell line. |
| Gene | ROCK1 |
| Host Cell | MCF7 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | (1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models |
| Size Form | >1 × 10^6 cells / vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Rho-associated kinase 1(ROCK1)regulates tumour metastasis by maintaining cytoskeletal homeostasis.Some researchers have investigated the function of ROCK1 in NSCLS survival using RNA interference-mediated knockdown.The results showed that ROCK1 knockdown reduced cell survival in vitro.It also inhibited cell migration and proliferation.Transfection of ROCK1 siRNA was associated with increased expression of large tumour suppressor kinase 2(LATS2)and c-Jun N-terminal kinase(JNK).In addition,ROCK1 knockdown-induced inhibition of apoptosis and proliferation was suppressed by LATS2 knockdown or JNK inactivation,suggesting that ROCK1 deficiency triggers apoptosis in a LATS2-JNK pathway-dependent manner.Functional analyses further showed that ROCK1 knockdown leads to dysregulation of mitochondrial dynamics and inhibits mitochondrial biogenesis.This effect was also reversed by LATS2 knockdown or JNK inactivation.
Figure 1.ROCK1 knockdown decreases cell migration and proliferation.(A)A CCK-8 assay was used to quantify proliferation in ROCK1 knockdown cells or control cells.(B,C)A qPCR assay was used to analyze Cyclin-D and Cyclin-E transcription.(D)Transwell assay for ROCK1 knockdown cells.(E,F)A qPCR assay was used to analyze CXCR-4 and CXCCR-7 transcription.(Xin,Tinget al.2020)
A: The ROCK1 gene encodes for the Rho-associated coiled-coil kinase 1, which is a serine/threonine kinase. ROCK1 plays a critical role in regulating the actin cytoskeleton, controlling cell motility, proliferation, and apoptosis. It is involved in various cellular processes, including smooth muscle contraction, cell adhesion, and epithelial-to-mesenchymal transition.
A: The ROCK1 gene contributes to disease development by its involvement in the regulation of cell processes. Dysregulation of ROCK1 has been linked to various conditions, including fibrosis, cancer metastasis, and cardiovascular diseases. Increased ROCK1 activity can promote aberrant cell behavior, leading to the progression of these diseases.
A: Targeting the ROCK1 gene has therapeutic implications for diseases where its dysregulation is a key factor. Inhibitors of ROCK1 activity can be used to treat conditions such as fibrosis, cancer metastasis, and hypertension. These targeted therapies can modulate ROCK1-dependent processes and potentially reverse or prevent the progression of these diseases.
A: The expression of the ROCK1 gene is regulated by various factors, including extracellular signals, cytokines, and transcription factors. It can be activated by Rho GTPases, such as RhoA, which are involved in cell signaling pathways. The activity of ROCK1 can also be influenced by post-translational modifications, such as phosphorylation, and its subcellular localization.
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The Human ROCK1 Knockdown Cell Line - MCF7 consistently demonstrates reliable and consistent results across various experiments, ensuring the validity and reproducibility of research findings.
The Human ROCK1 Knockdown Cell Line - MCF7 offers a cost-effective solution for researchers, providing a valuable tool for studying ROCK1 protein function without the need for expensive and time-consuming knockout techniques.
Researchers who have utilized the Human ROCK1 Knockdown Cell Line - MCF7 have reported positive feedback, highlighting its effectiveness in studying ROCK1-related cellular processes and its potential applications in drug discovery and development.
The provider of the Human ROCK1 Knockdown Cell Line - MCF7 offers excellent after-sales support, including detailed documentation, technical assistance, and prompt responses to inquiries, ensuring a smooth and hassle-free experience for researchers.
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