Human PRPF4B Knockout Cell Line-HEK293T

Name: Human PRPF4B Knockout Cell Line-HEK293T
SKU: CSC-RT3169
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT3169

Target Gene : PRPF4B Host Cell : HEK293T

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT3169
Introduction	This cell is a stable cell line with a homozygous knockout of human PRPF4B using CRISPR/Cas9.
Target Gene	PRPF4B
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

Prpf4b

Background

Predicted to enable protein serine/threonine kinase activity. Predicted to be involved in positive regulation of protein export from nucleus; regulation of intracellular signal transduction; and spliceosomal tri-snRNP complex assembly. Located in chromosome. Is expressed in several structures, including cerebral cortex; early conceptus; oocyte; and pancreas epithelium. Orthologous to human PRP4K (pre-mRNA processing factor kinase PRP4K). [provided by Alliance of Genome Resources, Feb 2025]

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