Human MST1 Knockdown Cell Line - HEK293

During apoptosis, caspases break and activate the serine/threonine kinase MST1 (mammalian STE20-like kinase 1). The purpose of the knockdown cell line was to investigate the function of MST1 in apoptosis. Although apoptotic chromatin condensation is known to be induced by MST1, the underlying mechanism is yet unknown. Their research showed that MST1 promotes chromatin condensation by phosphorylating histone H2AX at Ser-139. They illustrated MST1's function in H2AX phosphorylation using Western blots, in vitro kinase tests, and mutant H2AX proteins. Additionally, they demonstrated that MST1 and H2AX directly interact in HEK293 cells. H2AX was confirmed as an MST1 substrate in apoptosis by MST1 knockdown in Jurkat cells, which also decreased H2AX phosphorylation and DNA fragmentation after etoposide-induced apoptosis.

Figure 1. The researchers utilized MST1 knockdown Jurkat cells to investigate MST1's function in apoptosis. They administered etoposide treatment to the cells, and then used a variety of assays, such as cell lysate preparation and flow cytometry, to examine histone H2AX phosphorylation, DNA fragmentation, caspase-3 activity, and apoptosis. (Wen W, et al., 2010)

Creative Biogene's Human MST1 Knockdown Cell Line - HEK293 cell line can be used to study the role of MST1 in apoptosis. With this cell line, researchers can perform experiments similar to the above to verify the necessity of MST1 in histone H2AX phosphorylation, DNA fragmentation, and caspase-3 activity. Our cell line guarantees efficient knockdown of MST1 expression and is suitable for a variety of biological experiments, including drug treatment, protein expression detection, and flow cytometry analysis. Choosing Creative Biogene's MST1 knockdown cell line, you will benefit from our professional support, reliable cell quality, and fast experimental results. Contact us now to explore more experimental possibilities.