Human DDX60L Knockout Cell Line-A549

Name: Human DDX60L Knockout Cell Line-A549
SKU: CSC-RT3123
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT3123

Target Gene : DDX60L Host Cell : A549

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT3123
Introduction	This cell is a stable cell line with a homozygous knockout of human DDX60L using CRISPR/Cas9.
Target Gene	DDX60L
Host Cell	A549
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

DDX60L

Background

This gene encodes a member of the DExD/H-box helicase family of proteins, a subset of the super family 2 helicases. Members of the DExD/H-box helicase family share a conserved functional core comprised of two RecA-like globular domains. These domains contain conserved motifs that mediate ATP binding, ATP hydrolysis, nucleic acid binding, and RNA unwinding. In addition to functions in RNA metabolism, members of this family are involved in anti-viral immunity and act as cytosolic sensors of viral nucleic acids. The protein encoded by this gene has been shown to inhibit hepatitis C virus replication in response to interferon stimulation in cell culture. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]

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