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Cat. No. : PDPS-AR098
| Cat. No. | PDPS-AR098 |
| Description | Creative Biogene's Human Astrovirus (HAstV)Real Time RT-PCR Kit provides a ready-to-use system for the detection of Human Astrovirus (HAstV) using real-time RT-PCR (Reverse Transcription Polymerase Chain Reaction). |
| Species | virus |
| Features |
• High specificity • High accuracy • Fast & Simple & Efficient |
| Applications | Research Use |
| Nucleic Acid Type | RNA |
| Instrument |
• LightCycler 1.0, LightCycler 2.0 • PE5700, MJ-Opticon & other single color systems • ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX194, Lif96, Slan 96, iCycler iQ4, iCycler iQ5 & other multi-color systems |
| Note | For Research Use Only. Not for use in diagnostic procedures. |
| Storage |
• Store at -20°C • Repeated thawing and freezing (>101x) should be avoided • Cool all reagents during the working steps |
A: This kit is used to detect human Astrovirus by designing specific primers and probes based on the conserved sequence of HAstV and detecting the nucleic acid of HAstV by in vitro amplification using fluorescent PCR technique.
A: For blood samples, take 50 μl of anticoagulated lower blood cells and add 100 μl of double-distilled water and blow well. Subsequent RNA extraction can be performed if used immediately for the assay. Samples can be stored at -20°C for short term storage and at -70°C for long term storage, but no more than 6 months is recommended.
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All reagents required for real-time PCR are contained in one tube (i.e. Master Mix type), and only template RNA, Oligo Mix and DEPC-D.W need to be added during use to perform the reaction.
By minimizing non-specific reactions and maximizing reaction efficiency with Creative Biogene's Human Astrovirus (HAstV) Real Time RT-PCR Kit, only the target gene can be efficiently amplified, even with minute amounts of template RNA.
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