Transfected Stable Cell Lines
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Cat. No. : PDPS-AR203
| Cat. No. | PDPS-AR203 |
| Description | Creative Biogene's Helicobacter Pylori Real Time PCR Kit provides a ready-to-use system for the detection of Helicobacter Pylori using real-time RT-PCR (Reverse Transcription Polymerase Chain Reaction). |
| Species | bacteria |
| Features |
• High specificity • High accuracy • Fast & Simple & Efficient |
| Applications | Research Use |
| Nucleic Acid Type | DNA |
| Instrument |
• LightCycler 1.0, LightCycler 2.0 • PE5700, MJ-Opticon & other single color systems • ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX301, Lif96, Slan 96, iCycler iQ4, iCycler iQ5 & other multi-color systems |
| Note | For Research Use Only. Not for use in diagnostic procedures. |
| Storage |
• Store at -20°C • Repeated thawing and freezing (>208x) should be avoided • Cool all reagents during the working steps |
A: H. pylori is a bacterium that lives in stomachs of humans and animals. Helicobacter infection has been reported to cause chronic gastritis, gastric ulcer, duodenal ulcer, as well as gastric cancer. H. pylori is a bacterium that lives in a stomach where strong gastric juices are present. By breaking down the urea of the gastric mucosa into ammonia and carbon dioxide, and locally neutralizing the acid with the ammonia produced, its activity can settle down. Generally, it is confirmed through a biopsy or culture test to determine the presence of infection.
A: Helicobacter pylori Real Time PCR Detection Kit is designed for the detection of Helicobacter pylori in human or animal stool samples. After DNA insolation, the identification of Helicobacter pylori is performed by the use of target specific primers and a fluorescent-labeled probe that hybridizes to a conserved region with the ureB gene.
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All necessary reactants for real-time PCR are included in a tube (i.e., Master Mix Type), allowing the users to perform reaction simply by adding template DNA, Oligo Mix, and DEPC-D.W.
Creative Biogene's Helicobacter Pylori Real Time PCR Kits are highly sensitive, minimizing non-specific reactions and maximizing reaction efficiency so that only the target gene can be efficiently amplified, even with minute amounts of template DNA.
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