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PDPS-AR090

Genotype B5 of EnteroVirus 71(EV71-B5)Real Time RT-PCR Kit (RUO)

For research use only. Not intended for any clinical use.

Cat. No. :   PDPS-AR090

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Product Information

Cat. No. PDPS-AR090
Description Creative Biogene's Genotype B5 of EnteroVirus 71(EV71-B5)Real Time RT-PCR Kit provides a ready-to-use system for the detection of Genotype B5 of EnteroVirus 71(EV71-B5) using real-time RT-PCR (Reverse Transcription Polymerase Chain Reaction).
Species virus
Features • High specificity
• High accuracy
• Fast & Simple & Efficient
Applications Research Use
Nucleic Acid Type RNA
Instrument • LightCycler 1.0, LightCycler 2.0
• PE5700, MJ-Opticon & other single color systems
• ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX185, Lif96, Slan 96, iCycler iQ4, iCycler iQ5 & other multi-color systems
Note For Research Use Only. Not for use in diagnostic procedures.
Storage • Store at -20°C
• Repeated thawing and freezing (>92x) should be avoided
• Cool all reagents during the working steps
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Customer Reviews

Customer Q&As
What is the principle of the kit?

A: This kit uses polymerase chain reaction (PCR) technology combined with fluorescent probe technology to detect the specific nucleic acid fragment of enterovirus 71 subtype B5 by fluorescent PCR.

Does the kit have any requirements for the samples to be tested? Can it only be used for fresh samples?

A: The kit has a wide detection range and can be used to detect enterovirus 71 genotype B5 in nasopharyngeal secretions, sputum, induced sputum, stool, CSF, serum, etc. Fresh specimens collected can be used immediately for testing or stored at -70°C. However, repeated freezing and thawing of specimens should be avoided and low temperature shipping should be used, otherwise the test results may be affected.

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Customer Reviews
A Kit with Multiple Benefits

The kit uses gene-specific primers, reverse transcription and PCR reactions in a single tube, eliminating the need for additional tube opening/ pipetting, increasing throughput and reducing the risk of contamination.

United States

High Sensitivity

I used sputum as the test sample and detected enterovirus 71 genotype B5 in it with a sensitivity of up to 1 pg of total RNA.

United States

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