Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : PDPS-AR090
| Cat. No. | PDPS-AR090 |
| Description | Creative Biogene's Genotype B5 of EnteroVirus 71(EV71-B5)Real Time RT-PCR Kit provides a ready-to-use system for the detection of Genotype B5 of EnteroVirus 71(EV71-B5) using real-time RT-PCR (Reverse Transcription Polymerase Chain Reaction). |
| Species | virus |
| Features |
• High specificity • High accuracy • Fast & Simple & Efficient |
| Applications | Research Use |
| Nucleic Acid Type | RNA |
| Instrument |
• LightCycler 1.0, LightCycler 2.0 • PE5700, MJ-Opticon & other single color systems • ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX185, Lif96, Slan 96, iCycler iQ4, iCycler iQ5 & other multi-color systems |
| Note | For Research Use Only. Not for use in diagnostic procedures. |
| Storage |
• Store at -20°C • Repeated thawing and freezing (>92x) should be avoided • Cool all reagents during the working steps |
A: This kit uses polymerase chain reaction (PCR) technology combined with fluorescent probe technology to detect the specific nucleic acid fragment of enterovirus 71 subtype B5 by fluorescent PCR.
A: The kit has a wide detection range and can be used to detect enterovirus 71 genotype B5 in nasopharyngeal secretions, sputum, induced sputum, stool, CSF, serum, etc. Fresh specimens collected can be used immediately for testing or stored at -70°C. However, repeated freezing and thawing of specimens should be avoided and low temperature shipping should be used, otherwise the test results may be affected.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The kit uses gene-specific primers, reverse transcription and PCR reactions in a single tube, eliminating the need for additional tube opening/ pipetting, increasing throughput and reducing the risk of contamination.
I used sputum as the test sample and detected enterovirus 71 genotype B5 in it with a sensitivity of up to 1 pg of total RNA.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.