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Cat. No. : PDPS-AR209
| Cat. No. | PDPS-AR209 |
| Description | Creative Biogene's Enterobacter Sakazaii Real Time PCR Kit provides a ready-to-use system for the detection of Enterobacter Sakazaii using real-time RT-PCR (Reverse Transcription Polymerase Chain Reaction). |
| Species | bacteria |
| Features |
• High specificity • High accuracy • Fast & Simple & Efficient |
| Applications | Research Use |
| Nucleic Acid Type | DNA |
| Instrument |
• LightCycler 1.0, LightCycler 2.0 • PE5700, MJ-Opticon & other single color systems • ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX307, Lif96, Slan 96, iCycler iQ4, iCycler iQ5 & other multi-color systems |
| Note | For Research Use Only. Not for use in diagnostic procedures. |
| Storage |
• Store at -20°C • Repeated thawing and freezing (>214x) should be avoided • Cool all reagents during the working steps |
A: The kit contains enterobacter sakazakii-specific primers. When the sample contains enterobacter sakazakii, the enterobacter sakazakii DNA extracted after pre-enrichment is exponentially amplified by PCR. During the reaction, the enterobacter sakazakii-specific fluorescent probe hybridizes with the target nucleic acid and is simultaneously taq enzyme Hydrolysis to separate the fluorescent group from the quenching group on the probe: so that the presence of enterobacter sakazakii can be judged in real time by the fluorescence increment.
A: False negative results due to inhibition of the reaction can be excluded.
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The kit uses real time PCR technique to rapidly amplify nucleic acid sequences with high specificity and sensitivity.
The principle is based on the quantitative Real-time PCR technology and the kit provides specific primers and probes.The limit of detechion of target bacteria can reach 1-10CFU.
Pre-packed lysis buffer and Pre-mixed solution are provided. No extra reagents required. Multiple samples detections could run simultaneously. Detection process identifies results in 24 hours.
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