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PDPS-AR209

Enterobacter Sakazaii Real Time PCR Kit (RUO)

For research use only. Not intended for any clinical use.

Cat. No. :   PDPS-AR209

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Product Information

Cat. No. PDPS-AR209
Description Creative Biogene's Enterobacter Sakazaii Real Time PCR Kit provides a ready-to-use system for the detection of Enterobacter Sakazaii using real-time RT-PCR (Reverse Transcription Polymerase Chain Reaction).
Species bacteria
Features • High specificity
• High accuracy
• Fast & Simple & Efficient
Applications Research Use
Nucleic Acid Type DNA
Instrument • LightCycler 1.0, LightCycler 2.0
• PE5700, MJ-Opticon & other single color systems
• ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX307, Lif96, Slan 96, iCycler iQ4, iCycler iQ5 & other multi-color systems
Note For Research Use Only. Not for use in diagnostic procedures.
Storage • Store at -20°C
• Repeated thawing and freezing (>214x) should be avoided
• Cool all reagents during the working steps
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Q & A

Customer Reviews

Customer Q&As
How does the kit perform the test?

A: The kit contains enterobacter sakazakii-specific primers. When the sample contains enterobacter sakazakii, the enterobacter sakazakii DNA extracted after pre-enrichment is exponentially amplified by PCR. During the reaction, the enterobacter sakazakii-specific fluorescent probe hybridizes with the target nucleic acid and is simultaneously taq enzyme Hydrolysis to separate the fluorescent group from the quenching group on the probe: so that the presence of enterobacter sakazakii can be judged in real time by the fluorescence increment.

What is the purpose of the internal control provided in the kit?

A: False negative results due to inhibition of the reaction can be excluded.

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Customer Reviews
High Specificity and Sensitivity

The kit uses real time PCR technique to rapidly amplify nucleic acid sequences with high specificity and sensitivity.

United States

Precise and Reliable Results

The principle is based on the quantitative Real-time PCR technology and the kit provides specific primers and probes.The limit of detechion of target bacteria can reach 1-10CFU.

United States

Rapid Testing

Pre-packed lysis buffer and Pre-mixed solution are provided. No extra reagents required. Multiple samples detections could run simultaneously. Detection process identifies results in 24 hours.

United States

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