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Cat. No. : PDPS-AR241
| Cat. No. | PDPS-AR241 |
| Description | Creative Biogene's CP&MP Real Time PCR Kit provides a ready-to-use system for the detection of CP&MP using real-time RT-PCR (Reverse Transcription Polymerase Chain Reaction). |
| Species | pathogen |
| Features |
• High specificity • High accuracy • Fast & Simple & Efficient |
| Applications | Research Use |
| Nucleic Acid Type | DNA |
| Instrument |
• LightCycler 1.0, LightCycler 2.0 • PE5700, MJ-Opticon & other single color systems • ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX339, Lif96, Slan 96, iCycler iQ4, iCycler iQ5 & other multi-color systems |
| Note | For Research Use Only. Not for use in diagnostic procedures. |
| Storage |
• Store at -20°C • Repeated thawing and freezing (>246x) should be avoided • Cool all reagents during the working steps |
A: By using real time PCR systems, CP & MP real time PCR kit is used for the detection of chlamydia pneumoniae and mycoplasma pneumoniae in samples like nasal and pharyngeal secretions, sputum, provoked sputum, bronchial lavage, lung biopsy, pleural effusion and etc.
A: The kit is a specific ready-to-use system containing reagents and enzymes for the specific amplification of Chlamydia pneumoniae DNA. An external positive control is also included to determine gene load. For more information, please refer to the product specification.
A: Fluorescence is emitted and measured by the real time systems′ optical unit during the PCR. The detection of amplified MP DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. The detection of amplified CP DNA fragment is performed in fluorimeter channel 560nm with the fluorescent quencher BHQ1.
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Although this was my first time using the CP&MP Real Time PCR Kit, the kit provides an easy-to-use, ready-to-use system with detailed instructions for sample DNA extraction, PCR protocol, and data analysis. The first time I used the kit, I obtained the desired results quickly.
I use the kit to detect mycoplasma and chlamydia in samples in the laboratory. 96 samples can be detected simultaneously at one time, with high sensitivity and reproducible results, while greatly saving laboratory operation time.
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