AAV1-U6-shRNA-mCherry

Name: AAV1-U6-shRNA-mCherry
SKU: AAV00152Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAV00152Z

Serotype : AAV Serotype 1 Storage : -80 ℃

Titer: Size:

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Virus Particles Information

Quality Control

Cat. No.	AAV00152Z
Description	AAV serotype 1 particles contain scrambled shRNA under the control of U6 promoter and mCherry under CMV promoter.
Serotype	AAV Serotype 1
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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Background

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AAV is a small, nonenveloped virus with a single-stranded DNA genome of 4.7 kb containing two genes, rep and cap, located between two inverted terminal repeats. The rep gene produces the nonstructural Rep protein, which is essential for viral genome replication and packaging. The cap gene produces the three structural proteins, VP1, VP2, and VP3, which are translated from different start codons in a single open reading frame (ORF). Alternative mRNA splicing and the combined use of ATG codons and alternative start codons initiate VP protein translation, resulting in the proper capsid stoichiometry with a VP1/VP2/VP3 ratio of approximately 1:1:10.

The AAV genome was long believed to encode only Rep and VP proteins until 2010, when a second +1 frameshifted open reading frame (ORF) encoding a 204 amino acid long nonstructural protein was discovered in the cap gene of AAV serotype 2 (AAV2). This new AAV protein was named assembly-activating protein (AAP) for its role in capsid assembly. AAP2 is a nucleolar-localized protein essential for AAV1, -2, -8, and -9 capsid assembly.

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Customer Reviews

Excellent Fluorescent Marker

The mCherry fluorescent marker in AAV1-U6-shRNA-mCherry is remarkably bright and stable. It provides a clear and distinct visual confirmation of transduction efficiency, allowing for precise monitoring in our studies.

Germany

2022-07-07

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AAV1-U6-shRNA-mCherry

Virus Particles Information

Quality Control

Background

Q & A

Customer Reviews

Excellent Fluorescent Marker

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