Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : AAV00124Z
Serotype : AAV Serotype 1 Storage : -80 ℃
Titer: Size:
| Cat. No. | AAV00124Z |
| Description | AAV serotype 1 particles contain scrambled miRNA under the control of U6 promoter and GFP under the control of CMV promoter. |
| Serotype | AAV Serotype 1 |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Summary | Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
The production system of rAAV vectors usually consists of a DNA vector containing a transgene expression cassette flanked by inverted terminal repeats derived from AAV2. The construct size is limited to about 4.7-5.0 kb, which corresponds to the length of the wild-type AAV genome. rAAV is produced in a cell line. The expression vector is co-transfected with a helper plasmid that mediates the expression of the AAV rep gene, which is important for viral replication, and the cap gene, which encodes proteins that form the capsule. In addition, specific adenoviral genes required for replication are expressed in trans.
In recent years, a large number of naturally occurring AAV serotypes have been described, which differ mainly in the surface properties of the capsid. By combining an AAV2-based genome construct with a cap gene derived from a different serotype, so-called pseudotyped rAVVs can be generated. Currently, packaging systems for about 10 different serotypes are available for constructing vectors. Both AAV-1 and AAV-2-based vectors can transduce mouse liver and muscle in vivo; AAV-1 is more effective in muscle, while AAV-2 is more effective in liver.
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The packaging and delivery were prompt, and it was ready to use straight out of the vial. Highly recommend for anyone looking for a reliable vector for miRNA expression studies!
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