AAV1-TRE-GFP

Name: AAV1-TRE-GFP
SKU: AAV00143Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAV00143Z

Serotype : AAV Serotype 1 Storage : -80 ℃

Titer: Size:

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Cat. No.	AAV00143Z
Description	AAV serotype 1 particles contain GFP under TRE-Tight promoter and its expression can be responsive to the tTA and rtTA regulatory proteins.
Serotype	AAV Serotype 1
Reporter	GFP
Applications	1. Determination of optimal MOI (multiplicity of infection), administration methods etc. 2. Detection of the infection efficiency of the AAV serotype against a specific cell type or tissue. 3. Using reporter genes to visualize the distribution and expression of AAV vectors in live animals, helping assess the biodistribution and persistence of gene delivery.
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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In humans, AAV latent infection is common. However, AAV has never caused any pathogenic mechanisms. When AAV encounters a host cell, it enters the cell via receptor-mediated endocytosis. The capsid must then escape from the endosome and be transported to the nucleus where it is uncoated. The unique N-termini of both VP1 and VP2 contain nuclear localization signals required for capsid nuclear transport. In addition, VP1 contains a phospholipase domain, which is required for virion escape from endosomes.

Virions lacking VP1 are not infectious. After the capsid releases the genome, the genome must be converted to double-stranded form by Rep and the cellular DNA synthesis machinery. In the absence of a helper virus, wtAAV DNA can remain in a circular episomal form or integrate into the chromosome at the AAVS1 integration site. Both episomal and integrated DNA exist in a polysome-mediated ITR (inverted terminal repeat) form. In the presence of helper virus or cellular stress, AAV transcription and DNA replication are reactivated, and AAV completes its replication cycle.

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Customer Reviews

Robust GFP Expression

The clear GFP visualization facilitated the accurate quantification of transduction rates. The product definitely offers excellent value for the price. We will continue to order from Creative Biogene for future projects.

French

2022-08-01

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AAV1-TRE-GFP

Virus Particles Information

Quality Control

Background

Q & A

Customer Reviews

Robust GFP Expression

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