AAV1-CMV-saCas9-U6-sgRNA

Name: AAV1-CMV-saCas9-U6-sgRNA
SKU: AAV00127Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAV00127Z

Serotype : AAV Serotype 1 Storage : -80 ℃

Titer: Size:

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Virus Particles Information

Quality Control

Cat. No.	AAV00127Z
Description	AAV serotype 1 particles contain scrambled gRNA under the control of U6 promoter and saCas9 under CMV promoter.
Serotype	AAV Serotype 1
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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Adeno-associated virus (AAV)-based viral vectors are preeminent vehicles for viral vector-mediated transgenesis in animal models of physiological and pathological states, as well as for human gene therapy approaches. The prevalence of AAV as a vector stems from its low immunogenicity, high titers, no associated human disease, helper virus-free production, and high stability in terms of both duration of transgene expression in transduced cell and stability as a viral particle.

AAV serotype 1 (AAV1) is used in neuroscience applications such as optogenetics, pharmacogenetics, and gene silencing. AAV1 is also used in human clinical trials for the treatment of heart disease, alpha-1 antitrypsin deficiency, limb-girdle muscular dystrophy type 2D, Charcot-Marie-Tooth neuropathy, and Becker muscular dystrophy. The National Institutes of Health (NIH) classifies all serotypes of AAV-based vectors produced by helper-virus free methods as Risk Group 1, but depending on the transgene being expressed (e.g., oncogenes, toxic genes, etc.), the classification may be elevated.

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Customer Reviews

Versatile Application

Whether in vitro or in vivo, AAV1-CMV-saCas9-U6-sgRNA's applicability across different experimental models has saved us both time and resources.

Canada

2023-01-05

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AAV1-CMV-saCas9-U6-sgRNA

Virus Particles Information

Quality Control

Background

Q & A

Customer Reviews

Versatile Application

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