AAV1-CMV-RLuc

Name: AAV1-CMV-RLuc
SKU: AAV00147Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAV00147Z

Serotype : AAV Serotype 1 Storage : -80 ℃

Titer: Size:

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Cat. No.	AAV00147Z
Description	AAV serotype 1 particles contain firefly luciferase gene under CMV promoter.
Serotype	AAV Serotype 1
Reporter	RLuc
Applications	1. Determination of optimal MOI (multiplicity of infection), administration methods etc. 2. Detection of the infection efficiency of the AAV serotype against a specific cell type or tissue. 3. Using reporter genes to visualize the distribution and expression of AAV vectors in live animals, helping assess the biodistribution and persistence of gene delivery.
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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A decade ago, Friedman and Roblin proposed several barriers to gene therapy. Since then, adeno-associated virus (AAV) has emerged as a promising gene therapy vector that is being explored for use in numerous therapeutic applications. Wild-type AAVs are replication-incompetent parvoviruses (dependent viruses) that require a helper virus or cellular stress to replicate. They were discovered in the 1960s as contaminants in electron micrographs of adenoviruses and were thought to be satellite viruses.

These single-stranded DNA, non-enveloped viruses are approximately 24-26 nanometers in diameter. The virion consists of 60 capsid subunits that package a 4.7 kb genome that contains a 145-nucleotide inverted terminal repeat (ITR) at each end. ITRs are secondary structural elements that protect the linear, single-stranded genome, which has fewer than 10 unpaired nucleotides outside of the D sequence and contains regions required for AAV replication and packaging. Between the ITRs are the replication (Rep), capsid (Cap, composed of Vp1, Vp2, and Vp3), and assembly activation protein (AAP) open reading frames.

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The AAV1-CMV-RLuc product has consistently exceeded our expectations in our lab experiments. We’ve produced highly reproducible results, which is crucial for our studies.

Germany

2022-06-09

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AAV1-CMV-RLuc

Virus Particles Information

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