AAV1-CAG-Cre-mCherry

Name: AAV1-CAG-Cre-mCherry
SKU: AAV00125Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAV00125Z

Serotype : AAV Serotype 1 Storage : -80 ℃

Titer: Size:

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Cat. No.	AAV00125Z
Description	AAV serotype 1 particles contain Cre recombinase fused to mCherry under the control of CAG promoter.
Serotype	AAV Serotype 1
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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Many intermediate steps in the AAV wild-type infection cycle depend on specific interactions of capsid proteins with infected cells. These steps include initial recognition of surface receptors, induction of endocytosis, release from the endosomal compartment, transport to and entry into the nucleus, and final unpacking of viral DNA from the capsid. These interactions are critical determinants for efficient transduction and expression of target genes when rAAV is used as a gene delivery tool.

Indeed, significant differences in the transduction efficiency of various serotypes into specific tissues and cell types have been described. Therefore, in order to effectively express target genes, it is crucial to select the appropriate serotype. AAV1 is mainly expressed in the liver, followed by the heart and hindlimb skeletal muscles. AAV1 has been studied to treat conditions such as heart disease, alpha-1 antitrypsin deficiency, limb-girdle muscular dystrophy type 2D, Charcot–Marie–tooth neuropathy, and Becker muscular dystrophy.

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We have used the AAV1-CAG-Cre-mCherry across multiple projects, and it consistently delivers outstanding results.

United States

2023-11-30

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AAV1-CAG-Cre-mCherry

Virus Particles Information

Quality Control

Background

Q & A

Customer Reviews

Consistent Results

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