AAV DJ/8-mCherry

Name: AAV DJ/8-mCherry
SKU: AAV00232Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAV00232Z

Serotype : AAV serotype DJ/8 Storage : -80 ℃

Titer: Size:

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Virus Particles Information

Quality Control

Cat. No.	AAV00232Z
Description	AAV serotype DJ/8 particles contain mCherry under CMV promoter.
Serotype	AAV serotype DJ/8
Reporter	mCherry
Applications	1. Determination of optimal MOI (multiplicity of infection), administration methods etc. 2. Detection of the infection efficiency of the AAV serotype against a specific cell type or tissue. 3. Using reporter genes to visualize the distribution and expression of AAV vectors in live animals, helping assess the biodistribution and persistence of gene delivery.
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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Adeno-associated viruses (AAVs) have attracted a lot of attention in the gene therapy field because they have many properties that make them excellent vectors for gene transfer. Their genomes are easily manipulated, and AAV particles can be purified to very high titers and then lyophilized for easy handling/storage. AAVs can effectively transduce both proliferating and quiescent cells, and multiple serotypes with varying tropisms exist in nature, allowing for some degree of tissue targeting. In addition, there is general consensus that AAVs are likely to be safer than many other viral-based vectors because they are non-pathogenic and have relatively low innate immunogenicity.

Because genomic integration of AAV vectors is rare, the risk of insertional mutagenesis with AAV vectors is greatly reduced compared to retroviruses. These collective features have enabled AAV vectors to effectively correct a wide range of diseases in animal models, which in turn has led to a large number of clinical trials in the hope of safely achieving long-term expression of a variety of therapeutic proteins in human patients.

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Customer Reviews

Excellent results

The AAV DJ/8-mCherry's robust transduction efficiency in our in vivo experiments has significantly accelerated our research progress. The ease of use and reliable results have made it an indispensable tool in our studies.

United States

2022-03-31

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AAV DJ/8-mCherry

Virus Particles Information

Quality Control

Background

Q & A

Customer Reviews

Excellent results

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