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Cat.No. : CSC-RT0259
Host Cell: 293T Target Gene: UFC1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0259 |
| Description | 293T-UFC1 (-/-) is a stable cell line with a homozygous knockout of human UFC1 |
| Background | UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1 (UFM1; MIM 610553) (Komatsu et al., 2004 [PubMed 15071506]).[supplied by OMIM, Mar 2008] |
| Target Gene | UFC1 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
UFC1 Knockout Cell Line-293T has exceeded my expectations, delivering exceptional results in my genetic studies and providing a powerful tool for precise gene manipulation.
United States
04/11/2023
UFC1 Knockout Cell Line-293T's high efficiency and accuracy have allowed me to achieve targeted gene modifications with remarkable success rates, unlocking new insights into gene function.
Germany
08/09/2023
The easy to use protocol and comprehensive instructions have made the experimental process seamless, even for researchers with limited experience.
United States
08/23/2020
The reproducibility and reliability of the outcomes obtained using UFC1 Knockout Cell Line-293T have strengthened the scientific validity of my research findings.
United States
05/12/2021
I am grateful for the exceptional customer support provided by the manufacturer, who has been responsive, knowledgeable, and committed to ensuring the success of my gene knockout experiments.
France
08/15/2021
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