Cat.No. : CSC-RT0150
Host Cell: 293T Target Gene: UBXN6
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0150 |
| Description | 293T-UBXN6 (-/-) is a stable cell line with a homozygous knockout of human UBXN6 |
| Target Gene | UBXN6 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
UBXN6 Knockout Cell Line-293T has revolutionized my genetic studies, providing a powerful tool for precise gene manipulation and unravelling the mysteries of gene function.
I am highly impressed by the efficiency and effectiveness of UBXN6 Knockout Cell Line-293T, which consistently delivers successful gene knockouts with high accuracy.
The buyer-friendly protocol and clear instructions provided with UBXN6 Knockout Cell Line-293T have streamlined my experimental workflow, allowing me to focus more on the scientific aspects of my research.
The reproducibility and reliability of the results obtained using UBXN6 Knockout Cell Line-293T have bolstered the credibility of my findings, enhancing the impact of my research.
I deeply appreciate the exceptional quality and support provided by the manufacturer, who has been readily available to address any inquiries or issues I encountered during my gene knockout experiments.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
