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TMED4 Knockout Cell Line-293T

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0199

Target Gene :   TMED4 Host Cell :   293T

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No. CSC-RT0199
Description 293T-TMED4 (-/-) is a stable cell line with a homozygous knockout of human TMED4
Target Gene TMED4
Host Cell 293T
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Quick Inquiry

Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Accelerated progress

TMED4 Knockout Cell Line-293T has significantly accelerated my research progress, enabling in-depth investigations into the role of genes in various biological processes.

United States

Reproducibility

The reproducibility of results obtained with TMED4 Knockout Cell Line-293T has strengthened the validity and impact of my scientific findings.

United Kingdom

Opening up new avenues

TMED4 Knockout Cell Line-293T has transformed the way I approach gene manipulation, opening up new avenues for discovery and innovation.

United States

Advancing our understanding

TMED4 Knockout Cell Line-293T's contribution to advancing our understanding of gene function and disease mechanisms is profound.

United States

Elevated the quality

By utilizing TMED4 Knockout Cell Line-293T, I have elevated the quality and significance of my research, driving progress in my field.

United States

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