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QLK-L001

T7EI Digestion Kit

For research use only. Not intended for any clinical use.

Cat. No. :   QLK-L001

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Product Information

Cat. No. QLK-L001
Description This kit contains T7 Endonuclease I and buffer. T7 Endonuclease I recognizes and cleaves mismatch–specific DNA, even single base pair mismatches, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5' to the mismatch.
Applications Determine genome targeting efficiency by digesting annealed PCR products with T7 Endonuclease I, the most application is the Crispr/cas9 monitor test.
Storage Store at -20°C.
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Customer Q&As
What is T7 Endonuclease I?

A: T7 Endonuclease I is a junction-resolving enzyme of 149 amino acid residue, encoded by gene 3 of bacteriophage T7, existing as a stable dimer.

Is there a way to inactivate T7 Endonuclease I?

A: Yes, to inactivate T7 Endonuclease I we recommend using Proteinase K and incubating at 37°C for 5 minutes.

Does T7 Endonuclease I recognize single base pair mismatches?

A: Although T7 Endonuclease I is active against mismatched DNA, it is not an ideal enzyme to cleave all single-base pair mismatches in heteroduplex DNA.

What is the molecular weight of T7 Endonuclease I

A: The T7 Endonuclease I supplied is fused to the E. coli maltose binding protein. The molecular weight of the entire fusion protein is 60,302 daltons.

Can I use T7 Endonuclease I for genome editing applications?

A: Yes. T7 Endonuclease I can be used to detect indel formation (targeted editing events) in genome editing workflows.

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Customer Reviews
Worked very well

This kit can be used for gene mutation and SNP detection of TALEN and CRISPR/CAS9 results. I like it.

United States

Helpful tool

Great! Recognition and cleavage for non-perfectly matched DNA and Holliday junctions.

United Kingdom

User-friendly

High detection sensitivity and products can be directly detected by electrophoresis

United States

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