Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : QLK-L001
| Cat. No. | QLK-L001 |
| Description | This kit contains T7 Endonuclease I and buffer. T7 Endonuclease I recognizes and cleaves mismatch–specific DNA, even single base pair mismatches, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5' to the mismatch. |
| Applications | Determine genome targeting efficiency by digesting annealed PCR products with T7 Endonuclease I, the most application is the Crispr/cas9 monitor test. |
| Storage | Store at -20°C. |
A: T7 Endonuclease I is a junction-resolving enzyme of 149 amino acid residue, encoded by gene 3 of bacteriophage T7, existing as a stable dimer.
A: Yes, to inactivate T7 Endonuclease I we recommend using Proteinase K and incubating at 37°C for 5 minutes.
A: Although T7 Endonuclease I is active against mismatched DNA, it is not an ideal enzyme to cleave all single-base pair mismatches in heteroduplex DNA.
A: The T7 Endonuclease I supplied is fused to the E. coli maltose binding protein. The molecular weight of the entire fusion protein is 60,302 daltons.
A: Yes. T7 Endonuclease I can be used to detect indel formation (targeted editing events) in genome editing workflows.
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This kit can be used for gene mutation and SNP detection of TALEN and CRISPR/CAS9 results. I like it.
Great! Recognition and cleavage for non-perfectly matched DNA and Holliday junctions.
High detection sensitivity and products can be directly detected by electrophoresis
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