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Cat.No. : CSC-RT0310
Host Cell: 293T Target Gene: STRA13
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0310 |
| Description | 293T-STRA13 (-/-) is a stable cell line with a homozygous knockout of human STRA13 |
| Target Gene | STRA13 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
With its unparalleled effectiveness, STRA13 Knockout Cell Line-293T has become the go-to tool in my experiments, delivering precise gene modifications that translate into meaningful and interpretable data.
United States
08/23/2020
I appreciate the simplicity of its application, which has allowed me to quickly integrate it into my research projects, accelerating progress and providing a solid foundation for further investigations.
United States
05/12/2021
STRA13 Knockout Cell Line-293T's consistent performance has instilled a sense of confidence in my research outcomes, bolstering the validity of my findings and reinforcing the significance of my scientific contributions.
United Kingdom
08/15/2021
STRA13 Knockout Cell Line-293T's ease of use extends to both novice and experienced researchers, making it accessible to everyone in the lab and fostering a collaborative and productive research environment.
United States
10/09/2021
Time and again, STRA13 Knockout Cell Line-293T has demonstrated its reliability, consistently generating successful gene edits that have been key to unraveling the functional role of specific genes in various biological processes.
United States
01/27/2022
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