SELM Knockout Cell Line-293T

Name: SELM Knockout Cell Line-293T
SKU: CSC-RT0247
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0247

Target Gene : SELM Host Cell : 293T

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RT0247
Description	293T-SELM (-/-) is a stable cell line with a homozygous knockout of human SELM
Target Gene	SELM
Host Cell	293T
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

Q & A

Customer Reviews

The protein encoded by this gene belongs to the selenoprotein M/SEP15 family. The exact function of this protein is not known. It is localized in the perinuclear region, is highly expressed in the brain, and may be involved in neurodegenerative disorders. Transgenic mice with targeted deletion of this gene exhibit increased weight gain, suggesting a role for this gene in the regulation of body weight and energy metabolism. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3 UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Dec 2016]

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Efficient

SELM Knockout Cell Line-293T is a game-changer, offering an efficient and precise method for studying gene functions with remarkable success.

United States

2023-04-11

Indispensable

Its high knockout efficiency and user-friendly protocol make SELM Knockout Cell Line-293T an indispensable tool for researchers across various scientific disciplines.

United States

2020-08-23

Credibility

I am impressed by the consistent and reliable results obtained using SELM Knockout Cell Line-293T, which have contributed significantly to the credibility of my research.

United States

2021-05-12

Outstanding

The outstanding technical support provided by the manufacturer has been invaluable in addressing any queries or challenges during my experiments.

United States

2021-08-15

Recommend

I highly recommend SELM Knockout Cell Line-293T to fellow researchers, as it has undoubtedly enriched my scientific journey and advanced our understanding of genetics.

United States

2021-10-09

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SELM Knockout Cell Line-293T

Cell Line Information

Cell Culture Information

Safety and Packaging

Background

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Efficient

Indispensable

Credibility

Outstanding

Recommend

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