Panoply™ Human YES1 Knockdown Stable Cell Line

Name: Panoply™ Human YES1 Knockdown Stable Cell Line
SKU: CSC-DC017618
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC017618

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No.	CSC-DC017618
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	YES1
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	YES1 v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 [ Homo sapiens ]
Gene Symbol	YES1
Synonyms	Yes; c-yes; HsT441; P61-YES
Gene Description	v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
Gene ID	7525
Uni Prot ID	P07947
m RNA Refseq	NM_005433.3
Protein Refseq	NP_005424.1
Chromosome Location	18p11.31-p11.21
Function	ATP binding; enzyme binding; ion channel binding; non-membrane spanning protein tyrosine kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha-synuclein signaling, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; CD28 co-stimulation, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem;
MIM	164880

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Cervical cancer (CC) is a common malignant tumor affecting the female reproductive system. Its pathogenesis is complex and not only related to persistent infection with high-risk human papillomavirus (HR-HPV). YES1 is a non-receptor tyrosine kinase, but its specific impact on cervical cancer progression remains unclear. Here, researchers investigated the association between YES1 expression and the malignant biological characteristics of cervical cancer and further explored the potential pathogenic mechanisms mediated by YES1. The study showed that YES1 gene knockdown reduced cell proliferation, migration, and invasion abilities, as well as the expression of p62, Bcl-2, PCNA, and PI3K/AKT pathway proteins. Simultaneously, the expression of LC3II/I, Beclin-1, Bax, and cleaved Caspase-3, and the rate of apoptosis were increased. Therefore, YES1 gene knockdown has an inhibitory effect on cervical cancer, providing a theoretical basis for targeted therapy of cervical cancer.

Here, researchers used the CCK-8 assay to detect the effect of YES1 on the proliferation of SiHa and HeLa cells. The results showed that the proliferation ability of both YES1-knockdown SiHa and HeLa cells decreased (Figure 1A). Subsequently, the researchers performed colony formation assays in these two cervical cancer cell lines. The results showed that the colony formation ability of YES1-knockdown cells was reduced (Figure 1B). In addition, they also studied the effect of YES1 on the metastatic ability of cervical cancer cells using Transwell migration and wound healing assays. The wound healing assay results showed that the wound closure rate of YES1-knockdown cells decreased, indicating a decrease in cell migration ability (Figure 1C). In the Transwell migration assay, the researchers also observed a decrease in the number of cells that migrated through the Transwell membrane after YES1 downregulation. Furthermore, they used a Transwell invasion assay to study the effect of YES1 on the invasive ability of tumor cells. The results showed that after YES1 expression was downregulated, the number of cells that could penetrate the Matrigel and invade the lower chamber decreased. In summary, YES1 knockdown inhibited the invasion, migration, and proliferation abilities of cervical cancer cells.

Figure 1. YES1 down-regulation inhibits the invasion, migration, and proliferation of CC cells. (Feng N, Liu L., 2025)

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