Panoply™ Human TRPC5 Knockdown Stable Cell Line

TRPC5 belongs to the transient receptor potential (TRP) channel protein family and is typically expressed only in the brain. As a calcium channel protein, TRPC5 mediates the transport of extracellular free calcium into the cell. In this study, researchers investigated the effects of TRPC5 on the proliferation and invasion of papillary thyroid carcinoma (PTC). TRPC5 protein expression levels were higher in PTC tissues compared to adjacent normal thyroid tissues. TPC-1 cells overexpressing TRPC5 exhibited enhanced proliferative capacity, increased migration distance, and a higher number of invasive cells; conversely, TPC-1 cells with silenced TRPC5 expression showed reduced proliferation, shorter migration distances, and fewer invasive cells. Modulation of TRPC5 expression altered the levels of E-cadherin, Vimentin, MMP-9, MMP-2, and Twist, but did not affect the expression of ZEB or Snail. Xenograft experiments in nude mice demonstrated that inhibiting TRPC5 expression reduced both the volume and weight of the transplanted tumors. These results indicate that TRPC5 promotes the metastasis and progression of papillary thyroid carcinoma both in vitro and in vivo by upregulating the HIF-1α signaling pathway.

To investigate the role of TRPC5 silencing in TPC-1 cells, researchers generated TPC-1 cells with TRPC5 knockdown and verified the knockdown via RT-qPCR (Figure 1A). MTT cell viability assays revealed that, compared to the control group, the viability and proliferation rate of TRPC5-knockdown TPC-1 cells were inhibited (Figure 1B). Furthermore, compared to the control group, the migration count (Figures 1C and D) and migration distance (Figures 1E and F) of TRPC5-knockdown TPC-1 cells were also suppressed. Western blot analysis showed that, relative to the control group, TRPC5-knockdown TPC-1 cells exhibited reduced expression of Vimentin, MMP-2, TRPC5, and Twist, along with induced E-cadherin expression, whereas the expression of ZEB and Snail remained unaffected (Figures 1G and H). These results indicate that TRPC5 knockdown inhibits TPC-1 cell proliferation.

Figure 1. Silencing TRPC5 inhibits TPC-1 cell proliferation. (Yang J, et al., 2024)