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Panoply™ Human RFWD2 Knockdown Stable Cell Line

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-DC013202

Host Cell :   HEK293 (Hela and other cell types are also available) Validation :   Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No. CSC-DC013202
Description Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene RFWD2
Host Cell HEK293 (Hela and other cell types are also available)
Host Cell Species Homo sapiens (Human)
Applications

(1) Studying gene functions

(2) Studying gene interactions and signaling pathways

(3) Target validation and drug discovery

(4) Designing diseases models

Size >1 × 106 cells / vial
Stability Validated for at least 10 passages
Validation Real-Time RCR
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid Nitrogen
Shipping Dry Ice
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name RFWD2 ring finger and WD repeat domain 2, E3 ubiquitin protein ligase [ Homo sapiens ]
Gene Symbol RFWD2
Synonyms COP1; RNF200; RP11-318C24.3
Gene Description ring finger and WD repeat domain 2
Gene ID 64326
Uni Prot ID Q05CT6
m RNA Refseq NM_022457.5
Protein Refseq NP_071902.2
Chromosome Location 1q25.1-q25.2
Pathway Autodegradation of the E3 ubiquitin ligase COP1, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem; G1/S DNA Damage Checkpoints, organism-specific biosystem; Stabilization of p53, organism-specific biosystem; Ubiquitin mediated proteolysis, organism-specific biosystem;
MIM 608067
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