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Panoply™ Human RFC5 Knockdown Stable Cell Line

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-DC013189

Host Cell :   HEK293 (Hela and other cell types are also available) Validation :   Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No. CSC-DC013189
Description Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene RFC5
Host Cell HEK293 (Hela and other cell types are also available)
Host Cell Species Homo sapiens (Human)
Applications

(1) Studying gene functions

(2) Studying gene interactions and signaling pathways

(3) Target validation and drug discovery

(4) Designing diseases models

Size >1 × 106 cells / vial
Stability Validated for at least 10 passages
Validation Real-Time RCR
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid Nitrogen
Shipping Dry Ice
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name RFC5 replication factor C (activator 1) 5, 36.5kDa [ Homo sapiens ]
Gene Symbol RFC5
Synonyms RFC5; replication factor C (activator 1) 5, 36.5kDa; replication factor C (activator 1) 5 (36.5kD); replication factor C subunit 5; RFC36; A1 36 kDa subunit; RF-C 36 kDa subunit; RFC, 36.5 kD subunit; replication factor C, 36-kDa subunit; MGC1155;
Gene ID 5985
Uni Prot ID P40937
m RNA Refseq BC001866
Chromosome Location 12q24.3
Function ATP binding; DNA clamp loader activity; enzyme binding; nucleoside-triphosphatase activity; nucleotide binding; protein binding;
Pathway Activation of ATR in response to replication stress, organism-specific biosystem; BRCA1-associated genome surveillance complex (BASC), organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; DNA Repair, organism-specific biosystem;
MIM 600407
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