Panoply™ Human PIM3 Knockdown Stable Cell Line

Name: Panoply™ Human PIM3 Knockdown Stable Cell Line
SKU: CSC-DC011799
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC011799

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

Inquire for Price

Cell Line Information

Safety and Packaging

Gene Information

Cat. No.	CSC-DC011799
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	PIM3
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PIM3 pim-3 oncogene [ Homo sapiens ]
Gene Symbol	PIM3
Synonyms	pim-3
Gene ID	415116
Uni Prot ID	Q86V86
m RNA Refseq	NM_001001852.3
Protein Refseq	NP_001001852.2
Chromosome Location	22q13
Function	ATP binding; protein binding; protein serine/threonine kinase activity;
MIM	610580

Quick Inquiry

Case Study

Q & A

Customer Reviews

Melanoma is one of the most deadly types of common skin cancer, characterized by rapid proliferation and high invasiveness. Pim-3 is an oncogene highly expressed in melanoma and is a highly conserved serine/threonine kinase with various biological activities, such as promoting tumor cell proliferation and inhibiting apoptosis, thereby promoting cancer progression. Here, researchers found that knockdown of Pim-3 inhibited the migration and invasion of B16F10 cells in vitro. In a tumor-bearing mouse model, knockdown of Pim-3 significantly reduced lung metastasis of B16F10 melanoma cells in vivo. Mechanistic studies showed that knockdown of Pim-3 inhibited metastasis by regulating the expression of epithelial-mesenchymal transition (EMT)-related genes. Further research revealed that Pim-3 promotes the phosphorylation of STAT3 (signal transducer and activator of transcription 3), inducing the expression of Slug, Snail, and ZEB1, thereby enhancing EMT-related changes and inducing the migration and invasion of melanoma cells. These results suggest that Pim-3 is a potentially effective target for melanoma treatment.

To verify the effect of Pim-3 on B16F10 cell migration and invasion, researchers performed wound healing assays and Transwell migration assays in vitro. The wound healing assay results showed that compared with control cells, the migration ability of Pim-3 knockdown B16F10 cells was significantly reduced (Figure 1A, B). This change was independent of cell proliferation ability, as the proliferation rate of B16F10 cells did not change after transfection. Furthermore, silencing Pim-3 significantly reduced the migration ability of B16F10 cells (Figure 1C, D). Researchers used Boyden chambers pre-coated with Matrigel to detect the effect of Pim-3 on the invasion ability of B16F10 cells. Consistent with the migration assay results, knockdown of Pim-3 significantly reduced the number of cells that passed through the membrane (Figure 1E, F). These data indicate that Pim-3 plays an important role in promoting melanoma cell migration and invasion.

Figure 1. Knockdown of Pim-3 inhibits the migration and invasion of B16F10 cells in vitro. (Liu J, et al., 2018)

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *