Panoply™ Human P4HA2 Knockdown Stable Cell Line

Name: Panoply™ Human P4HA2 Knockdown Stable Cell Line
SKU: CSC-DC011215
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC011215

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No.	CSC-DC011215
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	P4HA2
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	P4HA2 prolyl 4-hydroxylase, alpha polypeptide II [ Homo sapiens ]
Gene Symbol	P4HA2
Gene Description	procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II
Gene ID	8974
Uni Prot ID	O15460
m RNA Refseq	NM_001017973.1
Protein Refseq	NP_001017973.1
Chromosome Location	5q31
Function	L-ascorbic acid binding; electron carrier activity; iron ion binding; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; procollagen-proline 4-dioxygenase activity;
Pathway	Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem;
MIM	600608

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