Panoply™ Human LIF Knockdown Stable Cell Line

Name: Panoply™ Human LIF Knockdown Stable Cell Line
SKU: CSC-DC008695
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC008695

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No.	CSC-DC008695
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	LIF
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	LIF leukemia inhibitory factor [ Homo sapiens ]
Gene Symbol	LIF
Synonyms	CDF; DIA; HILDA; MLPLI
GeneID	3976
Uni ProtID	P15018
mRNA Refseq	NM_002309.4
Protein Refseq	NP_002300.1
Chromosome Location	22q12.2
Function	cytokine activity; growth factor activity; leukemia inhibitory factor receptor binding; leukemia inhibitory factor receptor binding; receptor binding;
Pathway	Adipogenesis, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Direct p53 effectors, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; MicroRNAs in cardiomyocyte hypertrophy, organism-specific biosystem;
MIM	159540

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Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that promotes neuronal development and survival. Previous studies have shown that LIF mRNA expression is altered in nerve segments following peripheral nerve injury. Here, researchers found that LIF protein is highly expressed in Schwann cells after sciatic nerve injury in rats. Functionally, inhibiting or upregulating LIF increases or decreases, respectively, the proliferation rate and migratory capacity of Schwann cells. Morphological observations revealed that in vivo application of LIF-targeting siRNA following peripheral nerve injury promotes Schwann cell migration and proliferation, axonal elongation, and myelination. Electrophysiological and behavioral assessments indicated that LIF knockdown facilitates functional recovery of injured peripheral nerves. Differentially expressed LIF influences Schwann cell metabolism and negatively regulates ERFE (Erythroferrone). Collectively, these findings reveal the critical role of LIF in regulating Schwann cell proliferation and migration as well as the regeneration of injured peripheral nerves, identify ERFE as a downstream effector molecule of LIF, and broaden the understanding of the molecular mechanisms underlying peripheral nerve regeneration.

To evaluate the effect of LIF on Schwann cells, researchers generated LIF-knockdown Schwann cells (Figure 1A). EdU assay results showed that inhibiting LIF expression increased the number of EdU-positive Schwann cells and enhanced the cell proliferation rate (Figure 1B). Transwell migration assays demonstrated that LIF knockdown promoted the migration of Schwann cells across the Transwell chambers (Figure 1C). Similarly, wound healing assays revealed that, compared with control cells, LIF-knockdown Schwann cells exhibited a greater capacity to migrate into the wound area, resulting in a relatively smaller residual cell-free region (Figure 1D).

Figure 1. LIF affects Schwann cell proliferation and migration. (Chen Q, et al., 2021)

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