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Panoply™ Human ADHFE1 Knockdown Stable Cell Line

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-DC000305

Host Cell :   HEK293 (Hela and other cell types are also available) Validation :   Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No. CSC-DC000305
Description Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene ADHFE1
Host Cell HEK293 (Hela and other cell types are also available)
Host Cell Species Homo sapiens (Human)
Applications

(1) Studying gene functions

(2) Studying gene interactions and signaling pathways

(3) Target validation and drug discovery

(4) Designing diseases models

Size >1 × 106 cells / vial
Stability Validated for at least 10 passages
Validation Real-Time RCR
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid Nitrogen
Shipping Dry Ice
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name ADHFE1 alcohol dehydrogenase, iron containing, 1 [ Homo sapiens ]
Gene Symbol ADHFE1
Synonyms HOT; ADH8; HMFT2263
Gene Description alcohol dehydrogenase, iron containing, 1
Gene ID 137872
Uni Prot ID Q8IWW8
m RNA Refseq NM_144650.2
Protein Refseq NP_653251.2
Chromosome Location 8q13.1
Function hydroxyacid-oxoacid transhydrogenase activity; metal ion binding;
Pathway Interconversion of 2-oxoglutarate and 2-hydroxyglutarate, organism-specific biosystem; Metabolism, organism-specific biosystem; Pyruvate metabolism and Citric Acid (TCA) cycle, organism-specific biosystem; The citric acid (TCA) cycle and respiratory electron transport, organism-specific biosystem;
MIM 611083
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