Cat.No. : CSC-RT0237
Host Cell: 293T Target Gene: POLE3
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0237 |
| Description | 293T-POLE3 (-/-) is a stable cell line with a homozygous knockout of human POLE3 |
| Background | POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004] |
| Target Gene | POLE3 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Reflecting on my journey with POLE3 Knockout Cell Line-293T, I am filled with gratitude for the fruitful outcomes it has enabled, shaping my growth as a researcher in the field of genetics.
The support and resources provided by the manufacturer have been invaluable, helping me overcome experimental challenges and maximizing the potential of POLE3 Knockout Cell Line-293T in my studies.
Through using POLE3 Knockout Cell Line-293T, I have had the privilege of contributing to the advancement of genetic research, knowing that my findings have the potential to impact various fields of science.
As I continue to explore the vast landscape of genetics, I am confident that POLE3 Knockout Cell Line-293T will remain a steadfast companion, driving me towards exciting breakthroughs and illuminating new areas of scientific inquiry.
POLE3 Knockout Cell Line-293T has become more than just a tool in my lab; it symbolizes the spirit of exploration, curiosity, and the pursuit of knowledge that drives me as a dedicated scientist.
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