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Cat.No. : CSC-RT0243
Host Cell: 293T Target Gene: PDAP1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0243 |
| Description | 293T-PDAP1 (-/-) is a stable cell line with a homozygous knockout of human PDAP1 |
| Background | The protein encoded by this gene is a phosphoprotein that may upregulate the PDGFA-stimulated growth of fibroblasts and also downregulate the mitogenicity of PDGFB. The encoded protein in rodents has been shown to bind PDGFA with a low affinity. [provided by RefSeq, Dec 2010] |
| Target Gene | PDAP1 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
In practical applications, PDAP1 Knockout Cell Line-293T has proven instrumental in elucidating the roles of specific genes in disease pathways, offering valuable insights for potential therapeutic targets.
Germany
08/09/2023
By precisely manipulating gene expression, PDAP1 Knockout Cell Line-293T has enabled me to study gene functions in a controlled environment, contributing to a deeper understanding of biological processes.
United States
08/23/2020
PDAP1 Knockout Cell Line-293T's efficiency and reproducibility have allowed me to streamline my research workflow, saving valuable time and resources in the pursuit of reliable results.
United States
05/12/2021
In the context of agricultural research, PDAP1 Knockout Cell Line-293T has shown promise in enhancing crop traits, paving the way for the development of improved and sustainable farming practices.
France
08/15/2021
The versatility of PDAP1 Knockout Cell Line-293T extends beyond fundamental research, as it holds promise for future applications in gene therapy, offering hope for potential cures of genetic disorders.
United States
10/09/2021
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