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Cat.No. : CSC-RT0239
Host Cell: 293T Target Gene: NDUFS3
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0239 |
| Description | 293T-NDUFS3 (-/-) is a stable cell line with a homozygous knockout of human NDUFS3 |
| Background | This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.[provided by RefSeq, Apr 2009] |
| Target Gene | NDUFS3 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
As I peer through the microscope at the cells undergoing gene knockout, I am reminded of the transformative potential of NDUFS3 Knockout Cell Line-293T in unraveling the intricacies of life's blueprint.
United States
05/31/2022
The sense of wonder and excitement that accompanies each successful gene editing experiment serves as a constant reminder of the importance of perseverance in scientific exploration.
United States
04/01/2023
In the pursuit of knowledge, NDUFS3 Knockout Cell Line-293T NDUFS3 Knockout Cell Line-293T has been an essential ally, empowering me to pose new questions and seek answers in the vast universe of genetics.
United States
08/23/2020
From the initial stages of experimental design to the final analysis of data, NDUFS3 Knockout Cell Line-293T has been a reliable companion, guiding me through the twists and turns of the research process.
United States
05/12/2021
Beyond the confines of the laboratory, I am aware of the broader implications of my research, recognizing the potential of NDUFS3 Knockout Cell Line-293T to contribute to advancements in medicine and biotechnology.
United States
08/15/2021
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