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Cat.No. : CSC-RT0258
Host Cell: 293T Target Gene: NDUFA12
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0258 |
| Description | 293T-NDUFA12 (-/-) is a stable cell line with a homozygous knockout of human NDUFA12 |
| Background | This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012] |
| Target Gene | NDUFA12 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The collaboration opportunities facilitated by the manufacturer's research community have been valuable, allowing me to exchange ideas and learn from fellow researchers using the same gene knockout reagent.
United Kingdom
08/15/2021
The clear and concise data analysis guidelines provided by the manufacturer have been instrumental in interpreting the results of my gene knockout experiments accurately.
United States
10/09/2021
NDUFA12 Knockout Cell Line-293T's consistent availability and prompt restocking from the manufacturer have ensured uninterrupted progress in my research projects.
United States
01/27/2022
I have received positive feedback from my colleagues and peers about the impressive results I obtained using this gene knockout reagent, further reinforcing its reputation in the scientific community.
United States
07/19/2022
As I continue to explore new avenues in my research, I am confident that NDUFA12 Knockout Cell Line-293T will remain an indispensable tool in unlocking the genetic mysteries that lie ahead.
United States
11/03/2022
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