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Cat. No. : PDHS-CR013
| Cat. No. | PDHS-CR013 |
| Description | Creative Biogene's Mumps Virus Real Time RT-PCR Kit provides a ready-to-use system for the detection of Mumps Virus using real-time RT-PCR (Reverse Transcription Polymerase Chain Reaction). |
| Species | Virus |
| Features |
• High specificity • High accuracy • Fast & Simple & Efficient |
| Applications | Research Use |
| Note | For Research Use Only. Not for use in diagnostic procedures. |
| Storage |
• Store at -20°C • Repeated thawing and freezing (>3x) should be avoided • Cool all reagents during the working steps |
A: Mumps Virus real time RT-PCR kit is used for the detection of mumps virus by real time PCR systems in samples like nasal and pharyngeal secretions, sputum, serum, plasma, urine,C.S.F. and etc.
A: The principle of the real-time detection is based on the fluorogenic 5’nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially (Ct) is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities during Real Time allows the detection of the accumulating product without having to re-open the reaction tube after the amplificatio.
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The kit can be used with a variety of manual or automated sample preparation and assay setup platforms with good compatibility.
The primers and probes in Creative Biogene's Mumps Virus Real Time RT-PCR Kit have been optimized for high sensitivity. I recommend it.
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