MAPK9 Knockout Cell Line-293T

Name: MAPK9 Knockout Cell Line-293T
SKU: CSC-RT0459
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0459

Target Gene : MAPK9 Host Cell : 293T

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RT0459
Description	293T-MAPK9 (-/-) is a stable cell line with a homozygous knockout of human MAPK9
Target Gene	MAPK9
Host Cell	293T
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

Q & A

Customer Reviews

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this genes mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Sep 2008]

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Straightforward

Navigating the complexities of genetics has become a more straightforward and rewarding process, thanks to this reliable MAPK9 Knockout Cell Line-293T.

Germany

2021-10-09

Indispensable

MAPK9 Knockout Cell Line-293T is an indispensable tool that has revolutionized my genetic research, paving the way for groundbreaking discoveries.

United States

2022-01-27

Enhancing efficiency

MAPK9 Knockout Cell Line-293T has transformed my genetic research, enhancing its efficiency and effectiveness significantly.

United States

2022-07-19

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MAPK9 Knockout Cell Line-293T

Cell Line Information

Cell Culture Information

Safety and Packaging

Background

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Straightforward

Indispensable

Enhancing efficiency

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