Pages
Products

LAMTOR5 Knockout Cell Line-293T

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0279

Target Gene :   LAMTOR5 Host Cell :   293T

Size :   >1x106 cells/vial Validation :   Sequencing

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No. CSC-RT0279
Description 293T-LAMTOR5 (-/-) is a stable cell line with a homozygous knockout of human LAMTOR5
Background This gene encodes a protein that specifically complexes with the C-terminus of hepatitis B virus X protein (HBx). The function of this protein is to negatively regulate HBx activity and thus to alter the replication life cycle of the virus. [provided by RefSeq, Jul 2008]
Target Gene LAMTOR5
Host Cell 293T
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Quick Inquiry

Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Customer Reviews
Vital tool

In the pursuit of genetic understanding, LAMTOR5 Knockout Cell Line-293T emerges as a vital tool.

France

Efficacy

LAMTOR5 Knockout Cell Line-293T's efficacy in generating precise knockouts fuels my exploration of gene functions.

United States

Success

Consistent success underscores LAMTOR5 Knockout Cell Line-293T's role in advancing scientific inquiry and discovery.

United States

Facilitates

LAMTOR5 Knockout Cell Line-293T's versatility facilitates research in areas ranging from medicine to agriculture.

United States

Cornerstone

LAMTOR5 Knockout Cell Line-293T is a cornerstone of progress in the world of genetics.

United States

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Needs improvement

Satisfaction

General satisfaction

Very satisfaction