Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0167
Target Gene : KIF1C Host Cell : 293T
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0167 |
| Description | 293T-KIF1C (-/-) is a stable cell line with a homozygous knockout of human KIF1C |
| Background | The protein encoded by this gene is a member of the kinesin-like protein family. The family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. Mutations in this gene are a cause of spastic ataxia 2, autosomal recessive. [provided by RefSeq, May 2014] |
| Target Gene | KIF1C |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
KIF1C Knockout Cell Line-293T has exceeded my expectations, delivering exceptional results in my genetic studies and providing a powerful tool for precise gene manipulation.
I am impressed by the high efficiency and accuracy of KIF1C Knockout Cell Line-293T, enabling me to achieve targeted gene modifications with remarkable success rates.
The user-friendly protocol and straightforward workflow of KIF1C Knockout Cell Line-293T have simplified the experimental process, making it accessible to researchers with varying levels of expertise.
The reproducibility and reliability of the outcomes obtained using KIF1C Knockout Cell Line-293T have reinforced the robustness of my research findings, increasing their impact and significance.
I highly admire the outstanding technical support provided by the manufacturer, who has been responsive and knowledgeable, offering valuable assistance throughout my gene knockout experiments.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.