Cat.No. : CSC-RT0157
Host Cell: 293T Target Gene: ITPA
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0157 |
| Description | 293T-ITPA (-/-) is a stable cell line with a homozygous knockout of human ITPA |
| Background | This gene encodes an inosine triphosphate pyrophosphohydrolase. The encoded protein hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. This protein, which is a member of the HAM1 NTPase protein family, is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency which causes an accumulation of ITP in red blood cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012] |
| Target Gene | ITPA |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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ITPA Knockout Cell Line-293T has significantly advanced my genetic research, providing a reliable and efficient method for precise gene editing and deepening our understanding of gene function.
Right from the start,I am impressed by the high success rate of gene knockouts achieved with ITPA Knockout Cell Line-293T, enabling me to investigate the specific roles of genes with confidence and accuracy.
The simple and convenient user-friendly protocol and clear instructions accompanying ITPA Knockout Cell Line-293T have streamlined my experimental process, saving time and reducing errors.
The straightforward and convenient results obtained using ITPA Knockout Cell Line-293T have solidified the scientific integrity and reliability of my research outcomes.
I am thankful for the excellent customer support provided by the manufacturer, who has been readily available to answer my questions and provide guidance throughout my gene knockout experiments.
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