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Cat.No. : CSC-RT0050
Host Cell: HCT116 Target Gene: XRCC4
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0050 |
| Description | HCT116-XRCC4 (-/-) is a cell line with a homozygous knockout of human XRCC4 |
| Target Gene | XRCC4 |
| Host Cell | HCT116 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I think this brand's XRCC4 knockout cell line is very easy to use. Their cell line instructions are very detailed, so that I can easily carry out the experiment.
United States
01/12/2020
The customer service for this brand is always friendly and professional, answering my questions and providing support in a timely manner. I even had an abnormal experiment once and their customer service staff provided me with a solution quickly, which I really appreciated.
United States
04/11/2021
The cell lines are also very easy to store, and I can keep them in the refrigerator for a long time. In short, I strongly recommend this XRCC4 knockout cell line to other researchers, and I believe they can also benefit from it.
United States
11/21/2021
This brand's XRCC4 knockout cell line are fantastic! They've helped me obtain more accurate and reliable experimental data, and have greatly increased the efficiency of my experiments.
United States
01/31/2022
I've been searching for a high-quality and user-friendly gene knockout cell line, and I finally found it with this brand. I'm so grateful for the joy of successful experiments it has brought me!
United States
08/05/2022
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