Human TUBA1B Knockout Cell Line-HepG2

Name: Human TUBA1B Knockout Cell Line-HepG2
SKU: CSC-RT2089
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2089

Target Gene : TUBA1B Host Cell : HepG2

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2089
Description	This cell is a stable cell line with a homozygous knockout of human TUBA1B using CRISPR/Cas9.
Target Gene	TUBA1B
Host Cell	HepG2
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing
Storage	Liquid nirtogen

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TUBA1B tubulin, alpha 1b [ Homo sapiens ]
Gene Symbol	TUBA1B
Synonyms	K-ALPHA-1
Gene Description	tubulin, alpha 1b
GeneID	10376
Uni ProtID	P68363
mRNA Refseq	NM_006082.2
Protein Refseq	NP_006073.2
Chromosome Location	12q13.12
Function	GTP binding; GTPase activity; protein binding; structural constituent of cytoskeleton; structural molecule activity;
Pathway	Chaperonin-mediated protein folding, organism-specific biosystem; Cooperation of Prefoldin and TriC/CCT in actin and tubulin folding, organism-specific biosystem; Formation of tubulin folding intermediates by CCT/TriC, organism-specific biosystem; Gap junction, organism-specific biosystem; Gap junction, conserved biosystem; Metabolism of proteins, organism-specific biosystem; Pathogenic Escherichia coli infection, organism-specific biosystem;
MIM	602530

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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