Human SEPT9 Knockdown Cell Line-HeLa

Name: Human SEPT9 Knockdown Cell Line-HeLa
SKU: CSC-RK0287

Cat.No. : CSC-RK0287

Host Cell: HeLa Validation: Real-Time RCR

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Cell Line Information

Safety and Packaging

Cat. No.	CSC-RK0287
Gene	SEPT9
Alias	SEPT9, AF17q25, MSF, MSF1, NAPB, PNUTL4, SINT1, SeptD1, septin 9
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Morphology	Epithelial
Stability	Validated for at least 10 passages
Application	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size Form	>1 × 10^6 cells / vial
Shipping	Dry ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Septin-9 is a protein that in humans is encoded by the SEPT9 gene. SEPT9 has been shown to interact with SEPT2 and SEPT7. Along with AHNAK, eIF4E and S100A11, SEPT9 has been shown to be essential for pseudopod protrusion, tumor cell migration and invasion. The v2 region of the SEPT9 promoter has been shown to be methylated in colorectal cancer tissue compared with normal colonic mucosa. Using highly sensitive real time PCR assays, methylated SEPT9 was detected in the blood of colorectal cancer patients. This alternate methylation pattern in cancer samples is suggestive of an aberrant activation or repression of the gene compared to normal tissue samples

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