Human NOTCH2 Knockout Cell Line-HeLa

Name: Human NOTCH2 Knockout Cell Line-HeLa
SKU: CSC-RT0573
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0573

Target Gene : NOTCH2 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0573
Description	A stable cell line with a homozygous knockout of human NOTCH2 using CRISPR/Cas9.
Target Gene	NOTCH2
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing
Storage	Liquid nitrogen
Shipping	Dry ice package

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:4-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	NOTCH2
GeneID	4853

Quick Inquiry

Background

Applications

Q & A

Customer Reviews

NOTCH2 is one of four members of the NOTCH receptor family (which also includes NOTCH1, NOTCH3, and NOTCH4) and is an essential component of the highly conserved Notch signaling pathway. This pathway plays a critical role in various cellular processes, including cell fate determination, differentiation, proliferation, and apoptosis. The NOTCH2 gene is located on chromosome 1p12 and encodes a single-pass transmembrane protein that contains multiple functional domains, including EGF-like repeats, LIN-12/Notch repeats (LNRs), and an intracellular region involved in signal transduction.

Functionally, NOTCH2 signaling is essential for the development and homeostasis of multiple tissues, including the nervous, cardiovascular, and immune systems. It is particularly important in regulating kidney and liver development. Disruption or mutation of the NOTCH2 gene has been associated with various human pathologies, including Alagille syndrome and Hajdu-Cheney syndrome. NOTCH2 mutations have also been associated with malignancies, such as B-cell lymphomas and carcinomas, highlighting its role in tumorigenesis.

Cancer Research: The human NOTCH2 knockout cell line (HeLa) plays an important role in cancer research. By eliminating the NOTCH2 gene, researchers can study the role of this gene in cancer cell proliferation, survival, and metastasis.

Drug Screening: NOTCH2 knockout HeLa cells are used in high-throughput drug screening to evaluate new compounds that may inhibit cancer progression.

Pathway Analysis: These cells are essential for studying the NOTCH signaling pathway. Researchers can map the interactions and effects of NOTCH2 loss on cellular function, revealing how this pathway contributes to various physiological processes and diseases.

Genetic Studies: Using NOTCH2 knockout HeLa cells, genetic interactions and compensatory mechanisms in the absence of NOTCH2 can be explored.

Stem Cell Research: NOTCH2 plays a role in maintaining stemness in certain cell types. By using NOTCH2 knockout HeLa cells, researchers can study the specific contribution of this gene to stem cell renewal and differentiation, which is essential for developing regenerative medicine approaches.

Developmental Biology: Notch signaling is fundamental for cell differentiation during development. Therefore, NOTCH2 knockout models in HeLa cells can reveal developmental abnormalities and potential interventions.

Customer Q&As

What is the recommended growth medium? Does it require antibiotic selection?

A: DMEM supplemented with 10% fetal bovine serum. <br> It is not required to add the selection antibiotics when culturing the KO cells.

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Good experimental results

The NOTCH2 knockout cells lack functional NOTCH2 protein. Good experimental results were obtained.

United States

2023-04-11

Effectiveness

the Human NOTCH2 Knockout Cell Line-HeLa IS highly effective in elucidating the role of NOTCH2 in cellular processes. Its knockout efficiency and accuracy have provided valuable insights into developmental biology.

Germany

2021-05-12

Storage conditions

The Human NOTCH2 Knockout Cell Line-HeLa demonstrates exceptional stability under proper storage conditions. Its long shelf life ensures the availability of reliable cells for future experiments.

United States

2021-08-15

Quality

The Human NOTCH2 Knockout Cell Line-HeLa has become an invaluable tool in the study of NOTCH2-related diseases. Its exceptional quality and consistent results have gained recognition from the scientific community.

United States

2021-10-09

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Human NOTCH2 Knockout Cell Line-HeLa

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

What is the recommended growth medium? Does it require antibiotic selection?

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Good experimental results

Effectiveness

Storage conditions

Quality

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